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Add as Friendairlift fermenters

by: swati92

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Slide 1 : THE AIR LIFT FERMENTERS
Slide 2 : INTRODUCTION… Type of fermenters that are impeller-free systems. Employ forced/ pressurised air to circulate cells and nutrient medium. Can be used to culture cells that highly shear-sensitive.
Slide 3 : STRUCTURE/ DESIGN… Entire reactor is divided into 2 halves by a Draft tube: inner gassed region ( Riser) outer ungassed region ( Down comer) Riser has gas injection connected- air moves upwards. Down comer region has degassed media and cells. Mean density gradient between riser and downcomer regions causes continuous circulation.
Slide 4 :
Slide 5 : PARTS OF ALR… RISER: Connected gas injection- upward air flow. DOWNCOMER: degassed media+cells. BASE: Connected to Perforated nozzle bank/ plate/ Sparger to pump pressurized air. HEAD SPACE: Gas release region, flocculation, foam accumulation etc. GAS SEPARATOR: Facilitates gas/liquid recirculation maximizes gas residence time reduces gas friction in downcomer.
Slide 6 : Types of Gas separators in Internal loop ALRS
Slide 7 : Types of Gas separators in External loop ALRs
Slide 8 : TYPES OF ALRs….
Slide 9 : Internal Loop reactor External Loop reactor
Slide 10 : ADVANTAGES OF ALRs… Sparger has 2 functions: Aeration as well as Agitation. Hence, homogenous mixing/ distribution of gas/ oxygen. Homogenous distribution of nutrients and shear force. No focal points of energy/ heat dissipation- no agitator or stirrer. Key factors: Regulation of air bubble size Bubble residence time Liquid circulation and Gas injection.
Slide 11 : MODIFICATIONS OF ALRs…
Slide 12 : USES…. Mammalian cell cultures. Waste water treatment Biological processes involving biocatalysts as solids To produce biopharma proteins etc from fragile cells.
Slide 13 : THANK YOU…..

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