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    Add as Friendcell and tissue culture

    by: shoeb

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    1 : Cell And Tissue Culture Mammalian Cells Advanced Higher Biology
    2 : Why is it useful? Gene manipulation Culturing mammalian cells for cancer studies Producing new plants through tissue culture
    3 : What do you need to do it? Source of cell material -freshly prepared -stock of cell line -bacterial culture
    4 : Suitable container Simple flask Sophisticated fermenter with computer-controlled monitoring
    5 : Growth medium Glucose Water Amino acids Salts
    6 : Opportunity for Gas Exchange Oxygen Carbon dioxide
    7 : Animal serum Foetal Bovine Serum Essential for animal cell proliferation 5% - 10% of growth media
    8 : Indicator Waste products causes change in pH Use indicator like phenol red Changes from red to yellow
    9 : Control of Temperature and pH 37.5 OC pH 7.5
    10 : Method for Measuring Cell Growth Counting cell numbers in culture (haemocytometer) Measure optical density in spectrophotometer
    11 : Sterilisation Antibiotics Sterilisation
    12 : Cells are either…. Anchorage – dependant Anchorage - independant
    13 : Anchorage – independant cells Cells associated with body fluid -blood cells Grown in suspension Will eventually need subculturing
    14 : Anchorage – dependant cells Most animal derived cells Adhere to bottom of a flask and form a monolayer Eventually cover entire surface of substratum (confluence) Proliferation then stops Need to subculture cells at this point (remove to fresh medium) Proliferation can begin again
    15 : 2 main categories of animal cell cultures…. Primary culture Continuous cell line
    16 : Primary Cultures Taken from fresh tissue Limited life span in culture Treated by proteolytic enzyme (Trypsin) Separate into single cells -epithelial cells -fibroblasts
    17 : Continuous Cell Line Derived from humans Been transformed -lose sensitivity to factors associated with growth control Produce immortalised cell lines Cell lines are neoplastic Often lose their anchorage-dependence -associated with an altered xsome pattern More easily cultured
    18 :
    19 : Why do it? Particular cells can be isolated and cloned -isolation of mutant cells -investigate cell growth -produce hybrid cells that have applications in biotechnology Produce important pharmaceuticals -vaccines -hormones
    20 : This powerpoint was kindly donated to is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching.

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