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Slide 1 :
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BY
G.V.RAMANA REDDY
M PHARMACY 1st YEAR
DEPT.OF PHARMACEUTICAL ANALYSIS 1 Instrumentation and applications of gas chromatography |
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Instruments;
Carrier Gas
Flow regulators and meters
Sample injection system
Columns & ovens
Detectors
2 instrumentation |
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3 Schematic diagram of Gas Chromatograph |
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Gas Chromatograph Components Flame Ionization Detector Column Oven Injection Port top view front view |
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Carrier gas 5 The mobile phase gas is called the carrier gas and must be chemically inert.
Sample componet ? column ? detector
mobile phase gas
Helium ,argon ,nitrogen , carbon dioxide and hydrogen also used.
Selection of the best crrier gas very important , because it effects both the column separation and detector performance .
The ratio of viscosity of diffusion coefficient should be minimum for rapid analysis that’s why H, He are prepared for a carrier gas .
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Impurities in the carrier gas such as air water vapour and trace gaseous hydrocarbons can cause sample reaction, column character and affect the detector performance.
The carrier gas system should contains a molecular sieve to remove water and other impurities.
These gases are available in pressurized tanks. presure regulateres and flow meters are required to control the flow rate of the gas.
The gases are supplied from the high pressure gas cylinder , being stored at pressure up to 300psi
carrier gas should be better then 99.99%and 99.999% is often used 6 |
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